high fivetm cells in express fivetm medium (Thermo Fisher)
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high fivetm cells in express fivetm medium
High Fivetm Cells In Express Fivetm Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+fivetm+cells+in+express+fivetm+medium/high+fivetm+cells+in+express+fivetm+medium/pm40580950-317-188-193
Average 90 stars, based on 1 article reviews
High Fivetm Cells In Express Fivetm Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+fivetm+cells+in+express+fivetm+medium/high+fivetm+cells+in+express+fivetm+medium/pm40580950-317-188-193
Average 90 stars, based on 1 article reviews
high fivetm cells in express fivetm medium - by Bioz Stars,
2026-10
90/100 stars
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SDS Page:Article Title: Pseudouridine RNA avoids immune detection through impaired endolysosomal processing and TLR engagement. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti dsRNA Antibody, clone rJ2 Sigma-Aldrich Cat# MABE1134-25UL; RRID: AB_2819101 Anti mouse IgG, HRP linked antibody Cell Signaling Technology Cat# #7076; RRID: AB_330924 Chemicals, peptides, and recombinant proteins Acetonitrile Carl Roth Cat# HN40.1 Advanced RPMI 1640 medium Gibco Cat# 12633020 Ammonium persulfate Sigma-Aldrich Cat# A3678 BioColl Bio&Sell Cat# BS.L 6115 Blasticidin Thermo Scientific Cat# A1113903 CaptureSelectTM C-tagXL Affinity Matrix Thermo Scientific Cat# 2943072010 CD14 MircroBeads Human Miltenyi Cat# 130-050-201 Cellulose fibers Merck Cat#C6288-100G Coomassie Brilliant Blue R-250 Dye Thermo Scientific Cat# 20278 CU-CPT9a Invivogen Cat# inh-cc9a Express Five® SFM (1x) Gibco Cat# 10486025 Fetal calf serum Gibco Cat# 10270106 FuGene® Transfection Reagent Promega Cat# E2691 GlutaMAX Gibco Cat# 10270106 HEPES Sigma-Aldrich Cat# H0887-100ml vivo-jetRNA® VWR Cat#76579-662 LPS-EB Ultrapure InvivoGen Cat#tlrl-3pelps MEM NEAA Gibco Cat# 11140035 M5049 Invivogen Cat# inh-m5049 N1-Methylpseudouridine Sigma-Aldrich Cat# SMB01360 N1-Methylpseudo UTP Jena Bioscience Cat#NU-890S Opti-MEMTM Gibco Cat# 31985047 Penicillin/Streptomycin Gibco Cat# 15-140-122 Poly-L-arginine Sigma-Aldrich Cat#P7762 Pseudouridine Sigma-Aldrich Cat# SMB00912 Pseudo-UTP solution 100 mM Jena Bioscience Cat#NU-1139S Recombinant Human IL-3 MPI of Biochemistry, Munich N/A Recombinant Human CSF1 (M-CSF) MPI of Biochemistry, Munich N/A Recombinant Human RNase 1 MPI of Biochemistry, Munich N/A Recombinant Human RNase 2 MPI of Biochemistry, Munich N/A Recombinant Human RNase 6 MPI of Biochemistry, Munich N/A Recombinant Human TLR8 (AA27-827) This study N/A RBC lysis Buffer BioLegend Cat# 420301 RPMI 1640 medium Gibco Cat# 11875093 R848 InvivoGen Cat# tlrl-r848 SequaGel Concentrate National diagnostics Cat#EC830-1l SequaGel Buffer National diagnostics Cat# EC835-200ml SequaGel Diluent National diagnostics Cat# EC840-1l Sf-900TM III SFM Gibco Cat# 12658019 Sodium pyruvate Gibco Cat# 11360039 SYBR Gold Nucleic Acid Gel Stain Thermo Fisher Scientifc Cat# S33102 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, September 4, 2025 e1 Please cite this article in press as: Bé routi et al., Pseudouridine RNA avoids immune detection through impaired endolysosomal processing and TLR engagement, Cell (2025), https://doi.org/10.1016/j.cell.2025.05.032 Article .. 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Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti dsRNA Antibody, clone rJ2 Sigma-Aldrich Cat# MABE1134-25UL; RRID: AB_2819101 Anti mouse IgG, HRP linked antibody Cell Signaling Technology Cat# #7076; RRID: AB_330924 Chemicals, peptides, and recombinant proteins Acetonitrile Carl Roth Cat# HN40.1 Advanced RPMI 1640 medium Gibco Cat# 12633020 Ammonium persulfate Sigma-Aldrich Cat# A3678 BioColl Bio&Sell Cat# BS.L 6115 Blasticidin Thermo Scientific Cat# A1113903 CaptureSelectTM C-tagXL Affinity Matrix Thermo Scientific Cat# 2943072010 CD14 MircroBeads Human Miltenyi Cat# 130-050-201 Cellulose fibers Merck Cat#C6288-100G Coomassie Brilliant Blue R-250 Dye Thermo Scientific Cat# 20278 CU-CPT9a Invivogen Cat# inh-cc9a Express Five® SFM (1x) Gibco Cat# 10486025 Fetal calf serum Gibco Cat# 10270106 FuGene® Transfection Reagent Promega Cat# E2691 GlutaMAX Gibco Cat# 10270106 HEPES Sigma-Aldrich Cat# H0887-100ml vivo-jetRNA® VWR Cat#76579-662 LPS-EB Ultrapure InvivoGen Cat#tlrl-3pelps MEM NEAA Gibco Cat# 11140035 M5049 Invivogen Cat# inh-m5049 N1-Methylpseudouridine Sigma-Aldrich Cat# SMB01360 N1-Methylpseudo UTP Jena Bioscience Cat#NU-890S Opti-MEMTM Gibco Cat# 31985047 Penicillin/Streptomycin Gibco Cat# 15-140-122 Poly-L-arginine Sigma-Aldrich Cat#P7762 Pseudouridine Sigma-Aldrich Cat# SMB00912 Pseudo-UTP solution 100 mM Jena Bioscience Cat#NU-1139S Recombinant Human IL-3 MPI of Biochemistry, Munich N/A Recombinant Human CSF1 (M-CSF) MPI of Biochemistry, Munich N/A Recombinant Human RNase 1 MPI of Biochemistry, Munich N/A Recombinant Human RNase 2 MPI of Biochemistry, Munich N/A Recombinant Human RNase 6 MPI of Biochemistry, Munich N/A Recombinant Human TLR8 (AA27-827) This study N/A RBC lysis Buffer BioLegend Cat# 420301 RPMI 1640 medium Gibco Cat# 11875093 R848 InvivoGen Cat# tlrl-r848 SequaGel Concentrate National diagnostics Cat#EC830-1l SequaGel Buffer National diagnostics Cat# EC835-200ml SequaGel Diluent National diagnostics Cat# EC840-1l Sf-900TM III SFM Gibco Cat# 12658019 Sodium pyruvate Gibco Cat# 11360039 SYBR Gold Nucleic Acid Gel Stain Thermo Fisher Scientifc Cat# S33102 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, September 4, 2025 e1 Please cite this article in press as: Bé routi et al., Pseudouridine RNA avoids immune detection through impaired endolysosomal processing and TLR engagement, Cell (2025), https://doi.org/10.1016/j.cell.2025.05.032 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER TEMED Carl Roth Cat# 2367.3 TL8-506 InvivoGen Cat# tlrl-tl8506 TRIS glycine SDS-PAGE Thermo Scientific Cat# XP00125BOX Uridine Sigma-Aldrich Cat# U3750 β-Estradiol Sigma-Aldrich Cat#E8875 2x RNA loading dye Thermo Fisher Scientific Cat# R0641 5-Propargylamino-CTP-PEG5-AZDye488 Jena Bioscience Cat# NU-831-PEG5-AZ488 6x DNA loading dye Thermo Fisher Scienctific Cat#R1161 2 ′ ,3 ′ -cGMP BIOLOG Cat# G 025-250 Critical commercial assays Ampliscribe-T7-flash-transcription kit Lucigen Cat# ASF3507 Faustovirus Capping Enzyme New England Biolabs Cat# M2081l E. coli Poly(A) Polymerase New England Biolabs Cat# M0276l Human IFN-β ELISA Set R&D System Cat# DY814-05 Human IFN-α2 ELISA Set R&D System Cat# DY9345-05 Human IL-6 ELISA Set BD Biosciences Cat# 555220 Human TNF ELISA Set BD Biosciences Cat# 555212 Human IP10 ELISA Set BD Biosciences Cat# 550926 BCA protein assay Kit Thermo Fisher Scientific Cat# 23227 Mouse IFN-alpha ELISA Kit R&D Systems Cat# 42120-1 CD34 MicroBead Kit UltraPure, human Miltenyi Cat# 130-100-453 Monarch RNA Cleanup Kit New England Biolabs Cat# T2050l Experimental models: Cell lines BLaER1 human B-cell to monocyte trans-differentiation cell line Rapino et al 3.8 N/A CAL-1 Maeda et al. 39 N/A SF21 Thermo Fisher Scientific Cat#12682019 Bicinchoninic Acid Protein Assay:Article Title: Pseudouridine RNA avoids immune detection through impaired endolysosomal processing and TLR engagement. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti dsRNA Antibody, clone rJ2 Sigma-Aldrich Cat# MABE1134-25UL; RRID: AB_2819101 Anti mouse IgG, HRP linked antibody Cell Signaling Technology Cat# #7076; RRID: AB_330924 Chemicals, peptides, and recombinant proteins Acetonitrile Carl Roth Cat# HN40.1 Advanced RPMI 1640 medium Gibco Cat# 12633020 Ammonium persulfate Sigma-Aldrich Cat# A3678 BioColl Bio&Sell Cat# BS.L 6115 Blasticidin Thermo Scientific Cat# A1113903 CaptureSelectTM C-tagXL Affinity Matrix Thermo Scientific Cat# 2943072010 CD14 MircroBeads Human Miltenyi Cat# 130-050-201 Cellulose fibers Merck Cat#C6288-100G Coomassie Brilliant Blue R-250 Dye Thermo Scientific Cat# 20278 CU-CPT9a Invivogen Cat# inh-cc9a Express Five® SFM (1x) Gibco Cat# 10486025 Fetal calf serum Gibco Cat# 10270106 FuGene® Transfection Reagent Promega Cat# E2691 GlutaMAX Gibco Cat# 10270106 HEPES Sigma-Aldrich Cat# H0887-100ml vivo-jetRNA® VWR Cat#76579-662 LPS-EB Ultrapure InvivoGen Cat#tlrl-3pelps MEM NEAA Gibco Cat# 11140035 M5049 Invivogen Cat# inh-m5049 N1-Methylpseudouridine Sigma-Aldrich Cat# SMB01360 N1-Methylpseudo UTP Jena Bioscience Cat#NU-890S Opti-MEMTM Gibco Cat# 31985047 Penicillin/Streptomycin Gibco Cat# 15-140-122 Poly-L-arginine Sigma-Aldrich Cat#P7762 Pseudouridine Sigma-Aldrich Cat# SMB00912 Pseudo-UTP solution 100 mM Jena Bioscience Cat#NU-1139S Recombinant Human IL-3 MPI of Biochemistry, Munich N/A Recombinant Human CSF1 (M-CSF) MPI of Biochemistry, Munich N/A Recombinant Human RNase 1 MPI of Biochemistry, Munich N/A Recombinant Human RNase 2 MPI of Biochemistry, Munich N/A Recombinant Human RNase 6 MPI of Biochemistry, Munich N/A Recombinant Human TLR8 (AA27-827) This study N/A RBC lysis Buffer BioLegend Cat# 420301 RPMI 1640 medium Gibco Cat# 11875093 R848 InvivoGen Cat# tlrl-r848 SequaGel Concentrate National diagnostics Cat#EC830-1l SequaGel Buffer National diagnostics Cat# EC835-200ml SequaGel Diluent National diagnostics Cat# EC840-1l Sf-900TM III SFM Gibco Cat# 12658019 Sodium pyruvate Gibco Cat# 11360039 SYBR Gold Nucleic Acid Gel Stain Thermo Fisher Scientifc Cat# S33102 (Continued on next page) ll OPEN ACCESS Cell 188, 1–16.e1–e8, September 4, 2025 e1 Please cite this article in press as: Bé routi et al., Pseudouridine RNA avoids immune detection through impaired endolysosomal processing and TLR engagement, Cell (2025), https://doi.org/10.1016/j.cell.2025.05.032 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER TEMED Carl Roth Cat# 2367.3 TL8-506 InvivoGen Cat# tlrl-tl8506 TRIS glycine SDS-PAGE Thermo Scientific Cat# XP00125BOX Uridine Sigma-Aldrich Cat# U3750 β-Estradiol Sigma-Aldrich Cat#E8875 2x RNA loading dye Thermo Fisher Scientific Cat# R0641 5-Propargylamino-CTP-PEG5-AZDye488 Jena Bioscience Cat# NU-831-PEG5-AZ488 6x DNA loading dye Thermo Fisher Scienctific Cat#R1161 2 ′ ,3 ′ -cGMP BIOLOG Cat# G 025-250 Critical commercial assays Ampliscribe-T7-flash-transcription kit Lucigen Cat# ASF3507 Faustovirus Capping Enzyme New England Biolabs Cat# M2081l E. coli Poly(A) Polymerase New England Biolabs Cat# M0276l Human IFN-β ELISA Set R&D System Cat# DY814-05 Human IFN-α2 ELISA Set R&D System Cat# DY9345-05 Human IL-6 ELISA Set BD Biosciences Cat# 555220 Human TNF ELISA Set BD Biosciences Cat# 555212 Human IP10 ELISA Set BD Biosciences Cat# 550926 BCA protein assay Kit Thermo Fisher Scientific Cat# 23227 Mouse IFN-alpha ELISA Kit R&D Systems Cat# 42120-1 CD34 MicroBead Kit UltraPure, human Miltenyi Cat# 130-100-453 Monarch RNA Cleanup Kit New England Biolabs Cat# T2050l Experimental models: Cell lines BLaER1 human B-cell to monocyte trans-differentiation cell line Rapino et al 3.8 N/A CAL-1 Maeda et al. 39 N/A SF21 Thermo Fisher Scientific Cat#12682019 |